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. 2017 Aug 22;982:1–8. doi: 10.1016/j.aca.2017.06.020

Fig. 1.

Fig. 1

Analysis of human urine using gradient durations of 15, 7.5 or 3 min and column lengths of 150, 75 or 30 mm. The top chromatogram shows the BPI trace obtained from the UPLC-MS analysis of human urine using a 2.1 × 150 mm column and a gradient duration of 15 min, the centre chromatogram shows the same sample analysed using a 2.1 × 75 mm column and a gradient duration of 7.5 min, the lower chromatogram shows the same sample analysed using a 2.1 × 30 mm column and a gradient duration of 3 min.