Figure 3.

Loss of Cadm1 in excitatory neurons impacts body weight, energy homeostasis and thermogenesis. (a) Body weight curves of Slc17a6-Cre, Cadm1^flox/flox mice (n=12) and littermate controls (n=14) from age 4-12 weeks. (b) Glucose measurements during an ITT on 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=5) and control littermates (n=6). (c, d) Quantification of O₂ consumption, CO₂ production, RER, energy expenditure, locomotor activity, and food intake in 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=9) and littermate controls (n=10). (e) Energy expenditure of individual animals plotted against lean body mass from 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=9) and littermate controls (n=10). (f) Energy expenditure of individual animals plotted against locomotor activity in 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=9) and littermate controls (n=10). (g) Inguinal subcutaneous adipose tissue (iSAT) temperature measurements from 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=5) and control littermates (n=8) during cold challenge. (h) Brown adipose tissue (BAT) temperature measurements from 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=5) and control littermates after (n=8) during cold challenge. (i) Temperature measurements of BAT, iSAT and eye regions from 12-week old Slc17a6-Cre, Cadm1^flox/flox mice (n=5) and control littermates after (n=8) after one week exposure to cold. (j) Representative near-infrared analysis of 12-week old Slc17a6-Cre, Cadm1^flox/flox mice and control littermates after one week cold exposure. (k) Quantification of daily food intake of Slc17a6-Cre, Cadm1^flox/flox mice (n=5) and control littermates after (n=8) after cold exposure. All results are presented as mean ± s.e.m. *P<0.05 and **P<0.01. Boxplots show median, lower and upper quartiles (box), and minimum and maximum (whiskers). Statistical analyses are described in the Methods and Supplementary Table 3.