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. 2017 Apr 20;28(8):2459–2471. doi: 10.1681/ASN.2016060663

Figure 7.

Figure 7.

MiR-199a-5p or miR-214-3p shRNA lentiviruses blocked phenotypic transition and fibrosis in PD rats in vivo. (A) H&E and Masson staining, in situ hybridization showing the expression of miR-199a-5p and miR-214–3p, and immunohistochemistry showing the expression of α-SMA, E-cadherin, claudin-2, and FN proteins in HG-PDF rats treated with miR-199a-5p or miR-214–3p shRNA lentivirus compared with control lentivirus in vivo. U6 was tested as positive control and scramble probe was used as negative control. Original magnifications, ×100 (top panels) and ×400 (bottom panels). (B) The histogram shows the thickness of H&E staining in the submesothelial compact zone in miR-199a-5p shRNA (67.38±8.44) or miR-214–3p shRNA PD rats (71.00±9.41) compared with control lentivirus HG-PDF rats (121.50±5.57). Data are given as means±SEM (n=8). *P<0.05. (C and D) Peritoneal equilibration test of the quotient of dialysate and plasma concentration of urea nitrogen. Dialysate-to-plasma ratio of urea (D/P urea), and the initial dialysate–to–end dialysate ratio of glucose (D/D0 glucose), were used to evaluate the transport status of a PM in control rats, HG-PDF rats, and HG-PDF rats injected with cont, miR-199a-5p-shRNA, or miR-214–3p shRNA lentivirus. *P<0.05 versus control rats; **P<0.05 versus HG-PDF rats injected with control lentivirus. The detail data of D/P (urea) were: control rats 0.458±0.081, HG-PDF rats 0.735±0.054, cont-shRNA 0.708±0.057, miR-199a-shRNA 0.494±0.136, miR-214-shRNA 0.468±0.099; D/D0 glucose: control rats 1.055±0.270, HG-PDF rats 0.609±0.140, cont-shRNA 0.53±0.117, miR-199a-shRNA 0.825±0.207, miR-214-shRNA 0.795±0.195. cont, control; HE, hematoxylin-eosin.