Figure 6.

Nrip1 deficiency affects early pronephros marker gene expression in Xenopus embryos. (A–H) Pronephros marker gene analysis of Nrip1-depleted embryos at stage 22. Embryos were injected with nrip1 MO (+) or control MO (−) at the four-cell stage targeting their right sided pronephros. An in situ hybridization was performed for early pronephros markers (gata3, pax8, sall1, emx1, evi1, lhx1, tfap2b, and wt1) followed by the examination of their expression intensity on the injected (right) side compared with the uninjected side and quantified as shown in (I) (R, right side, injected; L, left side, uninjected). The expression of gata3 was not affected (P=0.16 z-test). The expression of pax8 (P=0.09) was slightly, but not significantly, reduced. Significant differences were observed for sall1 ([C]; P=0.035 z-test), evi1 ([D]; P=0.004), lhx1, tfap2b, and wt1 ([E–H]; P<0.001). n, number of embryos analyzed. *p<0.05; **p<0.01; ***p<0.001. coMO, control morpholino oligonucleotide; MO, antisense morpholino oligonucleotide; n.s., not significant.