Figure 2.

Nedd4L^Pax8/LC1 mice develop a K⁺ wasting phenotype after 2 weeks of LKD. (A and B) Plasma K⁺ and Na⁺ in control (white) and Nedd4L^Pax8/LC1 (gray) mice after 2 weeks of LKD. The renal suppression of NEDD4-2 results in severe hypokalemia and a slight increase in plasma [Na⁺] (n= 12 controls and five Nedd4L^Pax8/LC1 mice). (C and D) Analysis of 24-hour urinary K⁺ and Na⁺ excretion reveal elevated K⁺ excretion in mutant mice and concomitant but not significant Na⁺ retention (six mice in each group). (E and F) Plasma aldosterone (n=12 controls and five Nedd4L^Pax8/LC1 mice) and renin expression (n= five control and five Nedd4L^Pax8/LC1 mice) are decreased in Nedd4L^Pax8/LC1 mice as compared with controls. *P<0.05; **P<0.01.