Figure 5.

WNK1 and WNK4 are upregulated in Nedd4L^Pax8/LC1-deficient mice. (A and B) Western blot analysis of WNK1 and WNK4 in control and Nedd4L^Pax8/LC1 mice after 2 weeks of LKD, and protein quantification showing a significant increase in the two WNK proteins in Nedd4L^Pax8/LC1 mice (* indicates a nonspecific band; seven controls and five Nedd4L^Pax8/LC1 mice). (C and D) Costaining of NCC (red) with WNK1 (C) and WNK4 (D) (both in green) in control and Nedd4L^Pax8/LC1 mice. WNK1 and WNK4 foci are more prominent in the DCT of mutant versus control mice. (E and F) Costaining of WNK1 (green) and AQP2 (red) showing an increase in WNK1 apical localization in the CNT/CCD (E) (arrows) and MCD (F) (arrows) of mutant mice. *P<0.05; *P<0.01