Figure 1.

Variability for nine markers over 10 staining and stripping 2ME/SDS cycles. One single section for every three markers was stained (time 0) and sequentially stripped and restained for the same markers 10 times. Variation in staining intensity is expressed as a fraction of the 256 8bit channels over the initial staining intensity. Primary Ab incubation time: 1 hr, 2nd Ab: 30 min, single indirect IF. Data are from four independent 10-cycle experiments. Abbreviations: 2ME/SDS, beta-mercaptoethanol/sodium dodecyl sulfate; Ab = antibody; IF, immunofluorescence.