Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 10;65(8):431–444. doi: 10.1369/0022155417719419

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 2. — Comparison of antibody removal efficiency of 2ME/SDS and GnHCl. Tonsil sections were incubated with a FITC-conjugated anti-human IgM, stripped with either 2ME/SDS or GnHCl, counterstained with an AP-conjugated anti-FITC antibody and developed in NBT-BCIP. 2ME/SDS stripping (middle) leaves no stainable primary antibody. Stripping by GnHCl (bottom) leaves IgM+ plasma cells. Identical results are obtained with an anti-Rabbit AP-conjugated (not shown). High magnification in the insets. Scale bar: 500 µm. Abbreviations: 2ME/SDS, beta-mercaptoethanol/sodium dodecyl sulfate; GnHCl, guanidinium hydrochloride; FITC, Fluorescein Isothiocyanate; AP, alkaline phosphatase; NBT-BCIP, 5-bromo-4-chloro-3′-indolyl phosphate.