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. 2017 Jul 10;65(8):431–444. doi: 10.1369/0022155417719419

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Effect of stripping and restaining on abundant antigens. Sections in duplicate were stained in double indirect IF for the indicated abundant antigens (intermediate filaments). The channel value (A) or the % changes from the time 0 stain over 256 channels (B) ± SD are plotted before (Stain), after stripping (Strip), and after restaining with species- and isotype-matched nonimmune control Abs (Restain). Primary Ab incubation time: O/N, 2nd Ab: 30 min, double indirect IF. Abbreviations: Ab = antibody; IF, immunofluorescence.