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. 2017 Jul 10;65(8):431–444. doi: 10.1369/0022155417719419

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 9. — Detail of a multiplexed tonsil follicle. Six stains out of a 32-antibody multiplex are selected from a tonsil follicle and shown as single stains (inverted gray scale) on the left, or combined into four three-color RGB composites. Proliferating (Ki-67+) germinal center BCL6+ CD20+ B cells are mutually exclusive stained for IRF4. PD-1 stains CD3+ T cells in the germinal center as well as rare PD-1+, BCL6+, CD20+ centroblasts (white arrow). GnHCl stripping method. Scale bar = 100 µm. Abbreviations: RGB, red green blue additive color; GnHCl, guanidinium hydrochloride.