Fig. 5.

LPS-treated DCs modulate development and differentiation of T_reg subpopulations mediated by CD127 and 3G11. Splenocytes were isolated from mice which were i.v. transferred into LPS-treated DCs-MOG+LPS or DCs-MOG and DCs without incubation with LPS and MOG peptide shown in Fig. 3. Cells were re-stimulated with mouse IL-2 (1 ng/ml) and MOG peptide (0.1 µM) for 72 h at 37 °C. T lymphocytes were stained by anti-mouse CD4, CD25, CD127, GITR, FoxP3, and 3G11 antibodies. CD4⁺CD25⁺FoxP3⁺GITR⁺ T_regs were gated. Protein expression of 3G11 and CD127 on CD4⁺CD25⁺FoxP3⁺GITR⁺ T_regs was detected using flow cytometry. Error bars indicated in this figure represent mean and SD of triplicate determinations of frequencies of CD127⁺3G11⁺ (a) and CD127⁺3G11⁻ (b) regulatory T cell subpopulations in three independent experiments (*P < 0.05, n = 3, t test)