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. 2005 Feb 24;102(10):3788–3793. doi: 10.1073/pnas.0409773102

Fig. 1.

Fig. 1.

Akt activity is inhibited by gefitinib exclusively in NSCLC cell lines whose growth is inhibited by gefitinib. (A) Growth assays were performed on the cell lines shown while incubated in the presence or absence of gefitinib. Approximately 25,000–40,000 cells were seeded in 12-well plates and propagated in full serum (10% FBS) in the presence of gefitinib (1 μM) or vehicle control (DMSO). Cells were counted on day 5. Results are expressed as a percentage of control. Numerical depiction of the results is displayed below the graph. (B) Cell lines were grown in the presence of gefitinib (1 μM) or vehicle control (DMSO) for 6 h. Fifty micrograms of extract was probed with an antibody against phospho-Akt (Ser-473) and total Akt.