Skip to main content
. 2005 Feb 28;102(10):3840–3845. doi: 10.1073/pnas.0409777102

Fig. 1.

Fig. 1.

Highly resolved images of neurons grown on plastic tissue-culture dishes and expressing a variety of fluorescent proteins. (A) Spines (arrows) on dendrites of a cortical neuron transfected with dsRED. (×40, N.A. 0.60; scale bar, 15 μm.) (B) Cell bodies and neurites of a group of neurons containing CFP. (×10, N.A. 0.30; scale bar, 150 μm.) (C) Growth cones and neurites (arrows) on a striatal neuron expressing GFP. (×20, N.A. 0.45; scale bar, 50 μm.) (D) Cell bodies (arrows) of cortical neurons transfected with GFP. (×4, N.A. 0.13; scale bar, 300 μm.) (E) Images collected (×4) at approximately daily intervals after transfection demonstrate the ability to return to the same field of neurons. One neuron (open arrow) survives throughout the experiment; another (filled arrow) dies between days 4 and 5. (Scale bar, 300 μm.) (F) Intracellular and extracellular structures (e.g., neurites) of single neurons can be resolved and monitored over time. (×20, N.A. 0.45; scale bar, 60 μm.)