. 2017 Jul 28;12(7):e0181920. doi: 10.1371/journal.pone.0181920

Table 3. Comparison of the experimental design used in the previous and present studies to evaluate the role of the dipeptidyl peptidase IV and autolysin as virulence factors for S. suis serotype 2.

Experimental design	Previous study – Dipeptidyl peptidase IV [11]	Previous study – Autolysin [13]	This study
Sequence type (strain)	ST7 (05ZYH33)	ST378 (HA9801)	ST1 (P1/7) ST7 (SC84) ST25 (89–1591)
Adhesion to human fibronectin	Recombinant protein ELISA assay	Recombinant protein Western blot	Whole bacteria ELISA assay
Adhesion to epithelial cells	HEp-2 (Human laryngeal cells) MOI = 10 1 h or 2 h of incubation? % adhered inoculum	HEp-2 (Human laryngeal cells) MOI = 100 3 h of incubation % adhered inoculum? Adhesion of wild-type strain = 100%?	NPTr (Porcine tracheal cells) MOI = 10 2 h of incubation % adhered inoculum
Biofilm formation	Not evaluated	Microtiter plate assay after 24 h of incubation	Microtiter plate assay after 24 h of incubation
Virulence	Mouse Unknown (SPF), possibly BALB/c? Subcutaneous or intravenous? 1 x 10⁸ CFU/mouse?	Zebrafish Intraperitoneal 2 x 10³ to 2 x 10⁷ CFU/fish	Mouse C57BL/6 Intraperitoneal 5 x 10⁷ CFU/mouse