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. 2017 Jul 28;12(7):e0182381. doi: 10.1371/journal.pone.0182381

Fig 6. WRW4, a selective LXA4 receptor antagonist, reversed wound healing properties of LXA4-MS.

Fig 6

(A) qRT-PCR analysis was performed to assess the LXA4 receptor ALX mRNA’s abundance in skin ulcers collected on days 2, 7, and 14 in the control (vehicle—PBS/glue), Un-MS, soluble LXA4, and LXA4-MS groups. Data represent means ± SEM (n = 5 ulcers/group). One-way ANOVA was done to determine statistical significance (p < 0.05), which is indicated as follows: *, LXA4-MS vs. Vehicle (PBS/glue); #, LXA4-MS vs. Un-MS; and $, LXA4-MS vs. soluble LXA4. (B) Representative images of 1.5 cm dorsal wounds at days 2 and 7, with day 0 images serving as pre-injury images, are presented for the following groups: WRW4 (25 μl per animal—from a main peptide solution of 1 mg/ml), WRW4 + LXA4-MS (WRW4 applied 10 minutes before MS application– 10 mg of LXA4-MS), and LXA4-MS (10 mg). (C) Wound healing index values for the groups outlined in (B). Index values range from 0 to 1, where a value of 0 indicates the original wound, and a value of 1 represents a completely closed wound. Data represent means ± SEM (n = 9 ulcers/group); One-way ANOVA was done to determine statistical significance (*p < 0.05).