Table 2. Quantitative HPLC values and relative ratios from H2O2 oxidation, HI hydrolysis, and Soluene-350 analysis of samples relative to melanin controls.
Soluene-350 (/mg) | H2O2 Oxidat. (ng/mg) | HI Hydrolysis (ng/mg) | Ratios | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample | A500 | A650 | PTCA | PDCA | 4-AHP | 4-AHPEA | A650/A500 | PDCA/PTCA | 4-AHP /PTCA | 4-AHPEA/PDCA | 4-AHPEA/4-AHP | |
B. huntii orange | 0.200 | 0.037 | 8.49 | 25.1 | 9.58 | 1094 | 0.185 | 2.96 | 1.13 | 43.6 | 114.2 | |
B. melanopygus orange | 0.204 | 0.040 | 11.9 | 26.0 | 53.7 | 1870 | 0.196 | 2.19 | 4.53 | 72.1 | 34.9 | |
B. melanopygus black | 0.996 | 0.330 | 302 | 404 | 28.9 | 530 | 0.331 | 1.34 | 0.10 | 1.31 | 18.3 | |
B. impatiens/bimac. black | 0.861 | 0.263 | 252 | 378 | 96.1 | 228 | 0.305 | 1.50 | 0.38 | 0.60 | 2.4 | |
B. impatiens black T2+T3 | 0.750 | 0.232 | 210 | 338 | 7.4 | 260 | 0.309 | 1.61 | 0.04 | 0.77 | 35.3 | |
B. impatiens black T4+ | 0.898 | 0.296 | 301 | 429 | 14.4 | 372 | 0.330 | 1.42 | 0.05 | 0.87 | 25.8 | |
D. bioculata light orange | 0.119 | 0.020 | 18.6 | 49.7 | 5.74 | 350 | 0.168 | 2.67 | 0.31 | 7.04 | 61.0 | |
D. bioculata med orange | 0.072 | 0.014 | 5.9 | 22.3 | 5.5 | 198 | 0.194 | 3.80 | 0.94 | 8.90 | 36.0 | |
D. bioculata dark orange | 0.074 | 0.014 | 3.4 | 16.4 | 7.4 | 140 | 0.189 | 4.88 | 2.19 | 8.56 | 19.0 | |
D. occidentalis red | 0.220 | 0.045 | 8.4 | 13.1 | 22.9 | 782 | 0.205 | 1.55 | 2.73 | 59.9 | 34.1 | |
D. bioculata black | 0.268 | 0.062 | 55.9 | 91.2 | 3.7 | 283 | 0.231 | 1.63 | 0.07 | 3.10 | 76.4 | |
Dopa-melanin | 8.81 | 2.98 | 4790 | 5780 | 1110 | ND | 0.338 | 1.21 | 0.23 | 1/inf | 1/inf | |
DA-melanin | 5.96 | 1.67 | 2790 | 4750 | ND | 1200 | 0.280 | 1.70 | 1/inf | 0.25 | inf | |
Dopa+Cys-melanin | 4.79 | 0.63 | 4230 | 857 | 130000 | 867 | 0.132 | 0.20 | 30.7 | 1.01 | 0.01 | |
DA+Cys-melanin | 2.46 | 0.35 | 17.7 | 1940 | ND | 112000 | 0.142 | 110 | 1/inf | 57.7 | inf |
Colors reflect color of original sample taken from photographic RGB data.
For ease of interpretation, we highlighted cells based on similarity to melanin controls, with lighter shades indicating values in between melanin types.
inf = infinity.