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. 2017 Jul 28;12(7):e0182135. doi: 10.1371/journal.pone.0182135

Table 2. Quantitative HPLC values and relative ratios from H2O2 oxidation, HI hydrolysis, and Soluene-350 analysis of samples relative to melanin controls.

Soluene-350 (/mg) H2O2 Oxidat. (ng/mg) HI Hydrolysis (ng/mg) Ratios
Sample A500 A650 PTCA PDCA 4-AHP 4-AHPEA A650/A500 PDCA/PTCA 4-AHP /PTCA 4-AHPEA/PDCA 4-AHPEA/4-AHP
B. huntii orange 0.200 0.037 8.49 25.1 9.58 1094 0.185 2.96 1.13 43.6 114.2
B. melanopygus orange 0.204 0.040 11.9 26.0 53.7 1870 0.196 2.19 4.53 72.1 34.9
B. melanopygus black 0.996 0.330 302 404 28.9 530 0.331 1.34 0.10 1.31 18.3
B. impatiens/bimac. black 0.861 0.263 252 378 96.1 228 0.305 1.50 0.38 0.60 2.4
B. impatiens black T2+T3 0.750 0.232 210 338 7.4 260 0.309 1.61 0.04 0.77 35.3
B. impatiens black T4+ 0.898 0.296 301 429 14.4 372 0.330 1.42 0.05 0.87 25.8
D. bioculata light orange 0.119 0.020 18.6 49.7 5.74 350 0.168 2.67 0.31 7.04 61.0
D. bioculata med orange 0.072 0.014 5.9 22.3 5.5 198 0.194 3.80 0.94 8.90 36.0
D. bioculata dark orange 0.074 0.014 3.4 16.4 7.4 140 0.189 4.88 2.19 8.56 19.0
D. occidentalis red 0.220 0.045 8.4 13.1 22.9 782 0.205 1.55 2.73 59.9 34.1
D. bioculata black 0.268 0.062 55.9 91.2 3.7 283 0.231 1.63 0.07 3.10 76.4
Dopa-melanin 8.81 2.98 4790 5780 1110 ND 0.338 1.21 0.23 1/inf 1/inf
DA-melanin 5.96 1.67 2790 4750 ND 1200 0.280 1.70 1/inf 0.25 inf
Dopa+Cys-melanin 4.79 0.63 4230 857 130000 867 0.132 0.20 30.7 1.01 0.01
DA+Cys-melanin 2.46 0.35 17.7 1940 ND 112000 0.142 110 1/inf 57.7 inf

Colors reflect color of original sample taken from photographic RGB data.

For ease of interpretation, we highlighted cells based on similarity to melanin controls, with lighter shades indicating values in between melanin types.

inf = infinity.