Fig 3.

Presence of β-cell clusters (A -D) and islets (E & F) in mouse pancreas at gestational day (GD) 9. Immunohistochemistry was used to localize insulin (red), Glut2 (green), and Ki67 (white), whilst nuclei were counter-stained with DAPI (blue). Panels A-C show the same cluster to demonstrate insulin (A), Glut2 (B) and the merged image (C). Ins⁺Glut2^LO cells within clusters or islets without nuclear staining for Ki67 are indicated by the solid arrows in A-C and E. An Ins⁺Glut2^LO cell within a cluster showing nuclear staining for Ki67 is shown by the dashed arrow in D. Ins⁺Glut2^HI cells immunopositive for both Ki67 and Glut 2 can be seen in E and F (dashed arrows).