Fig 2. Expression of Arabidopsis FT gene in cassava.

The qRT-PCR results were obtained from four biological replicates and two technical replicates for each sample. 60444 represents the non‐transformed wildtype line and FT-02, FT-11, FT-13 and FT-17 represent the four independent transformants. The levels of detected amplification were normalized using 18S and Ubiquitin as reference genes. The expression cassette had an ethanol‐inducible promoter. In each case, potted cassava transgenic plants were either watered normally (H₂O), or the soil was drenched with 1% (v/v) ethanol for two weeks before leaves were harvested and analyzed.