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. 2017 Jul 14;13(7):e1006884. doi: 10.1371/journal.pgen.1006884

Fig 4. Cbfβ expression does not affect runx expression in the TG.

Fig 4

A-F, Colormetric in situ hybridization for runx3 (A-C) and cbfb (D-F) in the runx3w144/w144 and cbfbw128/w128 mutants focusing on the TG at 24hpf and 3dpf. G-J, Antibody staining of Elavl (green) to label the whole trigeminal in conjunction fluorescent in situ hybridization for runx3 (G-H) and cbfb (I-J) in runx3w144/w144 and cbfbw128/w128 mutants at 3dpf. K, Quantification of marker gene expression as total number of TG neurons/ganglion at 3dpf. Dashed line outlines the eye; Arrowhead, TG; X, vagal ganglion. Scale bar: A-F 100 μm, G-J 20μm. Embryos per condition (n = 3–7). All error bars represent S.E.M.