Skip to main content
. 2017 Jul 14;13(7):e1006888. doi: 10.1371/journal.pgen.1006888

Fig 2. Localization of NlpD fusions in ΔSSnlpD cells.

Fig 2

Overnight cultures of MT47 (ΔSSnlpD) harboring the integrated expression constructs (A) attHKNP20 (Plac::nlpDWT-mCherry), (B) attHKMT101 (Plac::nlpD(1–189)-mCherry), (C) attHKMT103 (Plac::nlpD(1–115)-mCherry), (D) attHKMT178 (Plac::ssdsbA-nlpD(102–175)-mCherry), (E) attHKMT180 (Plac::ssdsbA-nlpD(250–379)-mCherry), (F) attHKMT182 (Plac::ssdsbA-nlpD(189–379)-mCherry), or (G) attHKMT149 (Plac::nlpD(1–30)-mCherry) were diluted in minimal M9-maltose medium supplemented with 25μM (E-F), 50μM (A-B), 100μM (C-D), or 150μM (G) IPTG. Cells were grown at 30°C to an OD600 of 0.15–0.25 before they were visualized on 2% agarose pads by phase contrast and fluorescence microscopy. Arrows indicate localization of the protein fusion to division sites. Bar = 4μm.