Fig 6. NlpD and AmiC localize to division sites independently of YraP and/or the Tol-Pal system.
(A-D) Overnight cultures of (A) MT47 (ΔSSnlpD), (B) MT141 (ΔSSnlpD ΔyraP), (C) MT53 (ΔSSnlpD ΔtolQ-pal), or (D) MT147 (ΔSSnlpD ΔtolQ-pal ΔyraP) harboring the integrated expression construct attHKNP20 (Plac::nlpD-mCherry) were diluted in minimal M9-maltose medium supplemented with 100μM IPTG. (E-H) Overnight cultures of (E) TB143 (ΔamiC), (F) MT150 (ΔamiC ΔyraP), (G) MT149 (ΔamiC ΔtolQ-pal), or (H) MT178 (ΔamiC ΔtolQ-pal ΔyraP) harboring the integrated expression construct attHKNP16 (Plac-m3::amiC-gfp) were diluted in minimal M9-maltose medium supplemented with 25μM IPTG. For all strains, cells were grown at 30°C to an OD600 of 0.2–0.3 before they were visualized on 2% agarose pads by phase contrast and fluorescence microscopy. In all images, arrows indicate the localization of the protein fusion to division sites. Bar = 4μm.