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. 2017 Jul 14;13(7):e1006888. doi: 10.1371/journal.pgen.1006888

Fig 8. Recruitment of YraP to division sites is dependent on its OM localization but independent of NlpD, the Tol-Pal complex, and AmiC.

Fig 8

(A-C) Overnight cultures of MT140 (ΔyraP) harboring the integrated construct (A) attλMT197 (Plac::yraPWT-mCherry), (B) attλMT199 (Plac::yraPIM-mCherry), or (C) attλMT210 (Plac::ssdsbA-yraPperi-mCherry) were diluted in minimal M9-maltose medium supplemented with 10μM (A-C) or 20μM (B) IPTG. (D-G) Overnight cultures of (D) MT140 (ΔyraP), (E) MT141 (ΔyraP ΔSSnlpD), (F) MT147 (ΔyraP ΔSSnlpD ΔtolQ-pal), and (G) AAY22 (ΔyraP ΔamiC) harboring the integrated construct attλMT197 (Plac::yraPWT-mCherry) were diluted in minimal M9-maltose medium supplemented with 10μM IPTG. All cultures were grown at 30°C to an OD600 of 0.15–0.2 before cells were visualized on 2% agarose pads by phase contrast and fluorescence microscopy. Arrows indicate localization of the protein fusion to division sites. Bar = 4μm.