Fig 2. NRB4 also interacts with other βCAs.

(A) Plants with T-DNA insertions in βCA1 (βca1) and βCA2 (βca2) were tested for their response to benzothiadizole (BTH, a functional analog of SA), along with some control genotypes, double, and triple mutants. The response to BTH was measured based on weight, and plants were treated with either mock solution or 350 μM BTH four times over the course of three weeks, their weights recorded, and the ratio between the BTH and mock-treated plants calculated (15 plants in three groups of five). The ratio is expressed as percentage of fresh weight (%FW). (B) Structures of the βCAs mentioned in this work. The black rectangles show the coding sequence of each cDNA. The red rectangles show the predicted chloroplastic peptide (www.cbs.dtu.dk/services/ChloroP/). The βCAs were manually aligned with the beginning of the third exon of βCA1.1 as a reference. (C) Six additional βCAs were tested as in Fig 1A. (D) Eight additional βCAs were tested as in Fig 1A. In total, βCA1f, βCA2.2, βCA3.1, and βCA4.1 interacted with NRB4.