Capability of NSCs to differentiate into oligodendrocytes at 4, 7, and 14 days. (A) Quantitative analysis of MBP and SOX10 mRNA expression in the five culture groups under static conditions, n = 3. (B) Quantitative analysis of MBP and SOX10 mRNA expression in the NSC monolayer on the ECM and NSC spheroids in perfusion and static culture, respectively, n = 3. (C) Confocal images of the NSC monolayer on the ECM in static culture. Green represents SOX10 and blue is the nucleus. (D) Confocal images of NSC spheroids under perfusion conditions. Green represents SOX10 and blue is the nucleus. Scale bars represent 100 μm.