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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Neurosci Methods. 2017 May 26;287:1–12. doi: 10.1016/j.jneumeth.2017.05.023

Figure 1.

Figure 1

tGFP transfected PC-12 Adh cells. Representative images show PC-12 cells expressing tGFP fluorescent protein under (A) bright-field or (B) Green Fluorescent Protein filters. Cells were under constant selection using puromycin (passage 19). High transfection efficiency is evident, though it should be noted that there are varying degrees of signal intensity. (C) High magnification of a tGFP transfected cell showing perinuclear mitochondrial networks throughout the cytoplasm. Images A and B were taken on AMG scope, scale bar = 50μM. Image C taken on Nikon confocal Ti-e microscope using 488nm excitation laser, scale bar= 10μM.