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MTG label dissociates from mitochondria, leading to non-specific labeling. A. MTG labeling (green) of PC-12 cells after MTG mitochondria solution passage through 0.02 um filter. B. Absence of tGFP fluorescence after passing the tGFP mitochondria solution through 0.02um filter, indicating no leakage of the label. Scale bars= 50μM.
