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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Neurosci Methods. 2017 May 26;287:1–12. doi: 10.1016/j.jneumeth.2017.05.023

Figure 6.

Figure 6

Mitochondrial respiration changes with temperature and substrate addition. Mitochondrial respiration was highest when left on ice, with State III and V.1 decreasing significantly at room temperature (RT). Addition of substrates (subs) at RT helped maintain State III respiration. Bars are means ± SEM. *p<0.05 vs Ice; #p<0.05 vs Ice + subs (1-way ANOVA for each state, Tukey’s multiple comparison) n=3/group in triplicate.