Figure 6. Both modes of FtsY-membrane interactions impact efficient protein targeting.
(A) The effect of charge mutations in the αA1 motif on the co-translational targeting of pPL. Data were fitted to Equation 7. (B) Targeting efficiencies of the αA1 charge mutants correlate with the populations of molecules in the Dynamic mode in free FtsY. (C) The effect of A-domain truncations on the co-translational targeting of pPL. (D) Targeting efficiencies of A-domain truncation mutants parallel their abilities to sample the Stable mode prior to SRP binding. Values are reported as mean ± S.D., with n ≥ 3.
Figure 6—figure supplement 1. Co-translational protein targeting and translocation by FtsY mutants.
(A) Schematics of the co-translational targeting and translocation assay using trypsin-treated, high salt-washed endoplasmic reticulum membrane (TKRM). (B, C) Representative translocation data for FtsY charge-reversal (B) and truncation (C) mutants.