Figure 7. An engineered FtsY pre-organized into the Stable mode led to indiscriminate targeting.
(A, B) Time courses for targeting of RNCFtsQ (orange) and RNCLuc (blue) to SLB mediated by wildtype FtsY (A) and FtsY pre-organized into the Stable mode (B). Schematics of the single-molecule real-time targeting assay is depicted above. The amounts of FtsY in the two experiments were equalized by adjusting the surface density of tethered FtsY-dN1. (C) The amount of RNCFtsQ (orange bars) and RNCLuc (blue bars) targeted to SLB by tethered mutant FtsYs. (D) Targeting specificities of SLB-tethered FtsY mutants, defined by the ratio of targeted RNCFtsQ over RNCLuc in (C). Values are reported as mean ± S.D., with n ≥ 3.
Figure 7—figure supplement 1. The targeting of RNCFtsQ•SRP to SLB is dependent on FtsY.
The amount of RNCFtsQ•SRP targeted to SLB without or with membrane tethered FtsY-dN1 (+FtsY) were tested and quantified.