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a. In polysome profile/TrIP-Seq (transcript isoforms in polysome sequencing), defined fractionation according to the number of ribosomes on an mRNA dissects the mRNA pools under active translation and monitors differential translation status among mRNA isoforms.
b. TRAP (translating ribosome affinity purification) and Ribo-tag isolate ribosomes – including constituent mRNAs – from specific cells and lineages.
