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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Nat Methods. 2017 Jul 3;14(8):793–796. doi: 10.1038/nmeth.4347

Figure 2.

Figure 2

Tilting enables recovery of near-atomic resolution information from a dataset of the highly preferentially oriented HA trimer. (a) Top view, side view, and respective slices through the 3D reconstruction of the HA trimer from 40° tilted data. Representative glycan densities (red asterisks) are indicated. The separation of the (a-i) beta strands and (a-ii) alpha-helical pitch are well resolved if parallel to the direction of preferred orientation; (a-iii, a-iv) otherwise they are slightly less defined. (b) Map density of two alpha helices (residues 402-458 and residues 105-115) and (c) a beta sheet (residues 162-170, 200-215, 240-249). (d) Euler angle distribution of the reconstruction. (e) Slice through 3D FSC of the reconstruction thresholded at 0.143 cutoff, as well as the map-to-model 3D FSC thresholded at 0.5 cutoff. Sphericity of the 3D FSC is indicated. (f) Graphs showing the spread of 3D FSC values overlaid on global half-map and map-to-model curves.