Table 1.

List of TANC interactors.

TANC	Interacting protein			Experimental evidence	Ref.	TANC interaction region
	Name	Domain architecture	Pathway
1	α-internexin	Intermediate filament head, DNA-binding	Cytoskeleton organization	Co-IP	9	_
1	CAMKIIα	Kinase	Glutamate Receptor signaling	Co-IP	9	_
1	CASK	Casein Kappa	Glutamate Receptor signaling	Pull-down assay	9	_
2	CBY1	Chibby_fam	Wnt/Wingless signaling, Cilium assembly	SF-TAP/MS	32	_
2	CDC5L	myb- HTH DNA binding type 1 and 2, Myb/Cef1 domain	Spliceosome assembly	HTS AC-MS	76	_
2	CENPQ	CENP-Q domain, Coiled coil	Nucleosome assembly at the centromere	HTS AC-MS	77	_
2	CEP120*	2 C2, Coiled coil	Centrosome organization, Cilium assembly	HTS AC-MS	77	_
1	CEP128	Coiled coil	Centrosome organization, Cilium assembly	HTS AC-MS	78	_
1	CNTRL	4 LRR, 4 Coiled coil	Centrosome organization, Cilium assembly	HTS AC-MS	78	_
1	FBXW11	F-box, 7 WD repeats	Ubiquitin-mediated degradation	Co-IP	79	_
2	FMRP	Agenet-like, KH, FXMRP1_C_core, FXMR_C2	Regulation of translation	CLIP	83	_
1	Fodrin	23 Spectrin repeats, SH3,3 EF-hand	Cytoskeleton organization	Pull-down assay	9	ANK and TPR
1	GKAP	3 Coiled coil	Glutamate Receptor signaling	Co-IP	9	_
1	GluR1	TM	Glutamate Receptor signaling	Co-IP	9	_
1	GRIP	7 PDZ	Glutamate Receptor signaling	Pull-down assay	9	_
1	Homer	WH1/EVH1, Coiled coil	Glutamate Receptor signaling	Pull-down assay	9	_
2	INPP5E	13 repeats of P-X-X-P, Phosphatase	Cilium trafficking	SF-TAP/MS	32	_
2	LATS2	UB associated Kinase, AGC-kinase C-terminal	Hippo pathway	HTS PL-MS	74	_
2	MAPRE1	CH, EB1_C	Microtubule cytoskeleton regulation, Cilium assembly	SF-TAP/MS	32	_
1	MOV10	P-loop ATPase domain	RNA-mediated gene silencing	HTS AC-RNA	80	_
1	MINK	Kinase, CNH	Rap2-mediated signaling	Immunoblotting	75	TPR
1	NINL	4 EF-hand, 4 Coiled coil, KEN- box, D-box	Centrosome organization, Cilium assembly	HTS AC-MS	78	_
1	NR2B	Transmembrane receptor	Glutamate Receptor signaling	Co-IP	75	_
2	NR2C2	ZF- C4, NHR ligand binding	Nuclear receptor signaling pathways	HTS AC-MS	77	_
1	NXF1	RRM, 4 LLR repeats, NTF2, TAP-C	mRNA export from nucleus	HTS AC-RNA	80	_
2	PAK7	CRIB, kinase	Planar Cell Polarity pathway	HTS AC-MS	81	_
1	PCM1	Coiled coil, GTPase, molybdopterin domain	Centrosome organization, Cilium assembly	HTS AC-MS	78	_
2	PPP1CA	Ser/Thr phosphatase	Glutamate Receptor signaling, Hippo, Wnt signaling	HTS AC-MS	81	_
2	PPP1CC	Ser/Thr phosphatase	GluR, Hippo signaling	HTS AC-MS	81	_
1 & 2	PRICKLE1	PET, 3 LIMs	Planar Cell Polarity pathway	LC-MS/MS	72	_
1 & 2	PRICKLE2	PET, 3 LIMs	Planar Cell Polarity pathway	LC-MS/MS	72	_
1 & 2	PSD-95	3 PDZ, SH3, GK	Glutamate Receptor signaling	Y2H, Pull-down assay	8	LIG_PDZ_Class_1
1 & 2	SAP97	L27, 3 PDZ, SH3, GK	Glutamate Receptor signaling	Y2H, Pull-down assay	8	LIG_PDZ_Class_1
1	SCRIB	16 LRR repeats, 4 PDZ	Planar Cell Polarity pathway	SPR	65	LIG_PDZ_Class_1
1	SHANK1	6 ANK, SH3, PDZ, SAM	Glutamate Receptor signaling	Pull-down assay	9	_
2	SPIRE2	KIND, 3 WH2, ZF	Vescicle transport	HTS AC-MS	77	_
1	TNIK	Kinase, CNH	Rap2-mediated and Wnt signaling	Immunoblotting	75	TPR
2	XPO1 (CRM1)	Importin_N-term, 10 ARM/HEAT repeat like	Nuclear export	Pull down	82	_
2	YWHAB	14-3-3	Glutamate Receptor signaling, Hippo signaling	HTS AC-MS	74	_
2	ZYX	3 LIM, Zn binding	Hippo pathway	HTS AC-MS	81	_

For each interactor, the interacting TANC protein, the detection method and the binding region (experimentally validated) are here listed. Y2H: Yeast two hybrid; Co-IP: Co-immunoprecipitation; SPR: Surface plasmon resonance; HTS: High-Throughput System; AC: Affinity Capture; PL: Proximity Label; MS: Mass spectrometry; CLIP: Cross-Linking ImmunoPrecipitation; SF-TAP/MS: systematic tandem affinity purifications coupled to mass spectrometry. SLiMs are named according to the ELM nomenclature.