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. 2017 Jul 28;7:6773. doi: 10.1038/s41598-017-07185-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of TUDCA supplementation during embryo culture on the levels of intracellular reactive oxygen species (ROS) in porcine blastocysts. Representative fluorescence images of blastocysts from in vitro matured oocytes fertilized using ICSI with the conventional freeze-thaw (FT) boar spermatozoa without TUDCA-treatment (a), evaporatively dried boar spermatozoa without TUDCA-treatment (b) or evaporatively dried boar spermatozoa with 200 μM TUDCA-treatment (c). Bar: 100 μm. d. ROS contents were quantified as fluorescent intensity for three independent times. Each time 6–12 blastocysts were measured. Each bar represents the mean ± SD of the fluorescent density. Bars that do not share the same letter are significantly different at p < 0.05.