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. 2017 Jul 28;7:6773. doi: 10.1038/s41598-017-07185-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative transcript levels of pro-apoptotic gene of Bax and Bak and anti-apoptotic gene of Bcl-XL and Bcl-2 in blastocysts with or without TUDCA treatment. FT: Blastocysts developed from conventional freeze-thaw boar spermatozoa without TUDCA-treatment; ED: Blastocysts developed from evaporatively dried boar spermatozoa without TUDCA-treatment; ED+ TUDCA: Blastocysts developed from evaporatively dried boar spermatozoa with 200 μM TUDCA-treatment. mRNA levels of Bax and Bak in FT and ED+ TUDCA groups were normalized against those in ED group. GAPDH served as a loading control. Each bar represents the mean ± SD. All experiments were repeated for three times. Bars that do not share the same letter are significantly different at p < 0.05.