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. 2017 Jul 28;7:6773. doi: 10.1038/s41598-017-07185-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of TUDCA addition during embryo culture on the quality of porcine blastocysts. (a) Representative fluorescence images of TUNEL staining. Blue: DNA; Red: apoptotic cell; Bar: 30 μm. The indices of apoptosis (b) and fragmentation (c) are expressed as mean ± SD for four times. Different low case letters above columns indicate statistical differences at p < 0.05 by one way ANOVA followed by LSD test. FT: Blastocysts developed from conventional freeze-thaw boar spermatozoa without TUDCA-treatment; ED: Blastocysts developed from evaporatively dried boar spermatozoa without TUDCA-treatment; ED+ TUDCA: Blastocysts developed from evaporatively dried boar spermatozoa with 200 μM TUDCA-treatment.