Table 1.
Effect of different concentrations of TUDCA addition during embryo culture on the development of embryos.
| Groups* | No. of oocytes | Cleavage (%, mean ± SD) | 4–8 cell (%, mean ± SD) | >8 cell (%, mean ± SD) | Morula (%, mean ± SD) | Blastocyst (%, mean ± SD) |
|---|---|---|---|---|---|---|
| FT | 182 | 84.0 ± 1.9a | 75.3 ± 2.6a | 67.2 ± 2.3a | 50.8 ± 2.2a | 23.3 ± 2.4a |
| ED+ 0 μM | 184 | 74.7 ± 2.2b | 62.2 ± 2.6b | 52.4 ± 2.4b | 36.5 ± 1.7b | 15.5 ± 1.2b |
| ED+ 100 μM | 184 | 75.8 ± 2.0b | 64.3 ± 2.8b | 54.3 ± 1.4b | 37.1 ± 2.0b | 16.0 ± 2.0b |
| ED+ 200 μM | 185 | 81.3 ± 1.9c | 74.1 ± 1.3a | 65.5 ± 1.5a | 47.6 ± 1.7c | 19.6 ± 2.4c |
| ED+ 300 μM | 185 | 78.9 ± 2.1d | 68.3 ± 1.6c | 59.5 ± 2.3c | 37.5 ± 2.1b | 16.1 ± 1.9b |
Note: Different letters within the same column indicate statistical differences at p < 0.05 by one-way ANOVA followed by the LSD multiple comparison test (N = 8). FT: blastocysts developed from conventional freeze-thaw boar spermatozoa without TUDCA-treatment; ED+ 0 μM, ED+ 100 μM, ED+ 200 μM and ED+ 300 μM: blastocysts developed from evaporatively dried boar spermatozoa with 0, 100, 200 and 300 μM TUDCA-treatment, respectively.