Table 2.

Effect of TUDCA supplementation during embryo culture on the production of expanded and hatched blastocyst.

Groups	No. of blastocyst	Expanded blastocyst (%, mean ± SD)	Hatched blastocyst (%, mean ± SD)
FT	50	65.9 ± 2.9a	25.9 ± 2.9a
ED	48	60.3 ± 3.1b	21.1 ± 2.6b
ED+ TUDCA	50	74.1 ± 4.6c	35.9 ± 4.3c

Note: Different letters within the same column indicate statistical differences at p < 0.05 by one-way ANOVA followed by the LSD multiple comparison test (N = 5). FT: blastocysts developed from conventional freeze-thaw boar spermatozoa without TUDCA-treatment; ED and ED+ TUDCA: blastocysts developed from evaporatively dried boar spermatozoa with 0 and 200 μM TUDCA-treatment, respectively.