Table 3.

Effect of TUDCA supplementation during embryo culture on the quality of blastocysts.

Groups	No. of blastocyst	ICM (mean ± SD)	TE (mean ± SD)	Total (mean ± SD)	ICM/Total (%, mean ± SD)
FT	30	5.1 ± 0.1a	25.9 ± 0.4a	31.0 ± 0.4a	16.6 ± 0.4a
ED	30	3.6 ± 0.1b	21.4 ± 0.4b	25.0 ± 0.4b	14.4 ± 0.5b
ED+ TUDCA	30	7.0 ± 0.2c	27.5 ± 0.1c	34.5 ± 0.3c	20.3 ± 0.3c

Note: Different letters within the same column indicate statistical differences at p < 0.05 by one-way ANOVA followed by the LSD multiple comparison test (N = 3). FT: blastocysts developed from conventional freeze-thaw boar spermatozoa without TUDCA-treatment; ED and ED+ TUDCA: blastocysts developed from evaporatively dried boar spermatozoa with 0 and 200 μM TUDCA-treatment, respectively. ICM: cell numbers of the inner cell mass; TE: numbers of trophoblast cells; Total: cell numbers of the inner cell mass plus numbers of trophoblast cells.