Table 4.
Gene | Primers (5′-3′) | Product size (base pair) | RT-PCR condition |
---|---|---|---|
BAX | F: AAGCGCATTGGAGATGAACT R: GGCCTTGAGCACCAGTTTAC | 184 | 40 cycles/57 °C |
BAK | F: CTGCCCCTAGAACCTAGCAG R: TTGATGCCACTCTCGAACAG | 186 | 40 cycles/57 °C |
BCL-2 | F: ATGTGTGTGGAGAGCGTCAA R: CCTTCAGAGACAGCCAGGAG | 188 | 45 cycles/57 °C |
BCL-XL | F: ACAGCGTATCAGAGCTTTGAGCA R: CGTCAGGAACCATCGGTTGAAG | 297 | 35 cycles/58 °C |
GAPDH | F: TTCCACGGCACAGTCAAGGC R: CATGGTCGTGAAGACACCAG | 151 | 40 cycles/57 °C |
Note: F: forward primer; R: reverse primer.