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. 2017 Jul 28;7:6773. doi: 10.1038/s41598-017-07185-w

Table 4.

Primers used for RT-PCR analyses.

Gene Primers (5′-3′) Product size (base pair) RT-PCR condition
BAX F: AAGCGCATTGGAGATGAACT R: GGCCTTGAGCACCAGTTTAC 184 40 cycles/57 °C
BAK F: CTGCCCCTAGAACCTAGCAG R: TTGATGCCACTCTCGAACAG 186 40 cycles/57 °C
BCL-2 F: ATGTGTGTGGAGAGCGTCAA R: CCTTCAGAGACAGCCAGGAG 188 45 cycles/57 °C
BCL-XL F: ACAGCGTATCAGAGCTTTGAGCA R: CGTCAGGAACCATCGGTTGAAG 297 35 cycles/58 °C
GAPDH F: TTCCACGGCACAGTCAAGGC R: CATGGTCGTGAAGACACCAG 151 40 cycles/57 °C

Note: F: forward primer; R: reverse primer.