Skip to main content
. 2017 Jul 28;7:6847. doi: 10.1038/s41598-017-07126-7

Figure 3.

Figure 3

c-Myb and LEF1 are responsible for DDR2 promoter activity. (a) Schematic representation of the DDR2 promoter and possible relevant transcription factors (top). Relative expression of a mock or DDR2 wild-type (WT) promoter-driven luciferase reporter in H1299 cells incubated on a PAG with the indicated stiffness or on glass (bottom). (b) Western blot analysis of green fluorescent protein (GFP) under the control of the DDR2 WT promoter in H1299 cells incubated on a PAG with the indicated stiffness. (c) A schematic of the DDR2 WT promoter and six truncated constructs in reporter vectors (top). Relative expression of DDR2 WT or truncated promoter–driven luciferase reporters in H1299 cells incubated on a PAG with the indicated stiffness (bottom). (d) Relative expression of DDR2 WT promoter–driven luciferase reporters in FOXA1-, c-Myb-, ELF1-, or LEF1-overexpressing H1299 cells (bottom). (e,f) Relative expression of DDR2 WT promoter–driven luciferase reporters in c-Myb- or LEF1-overexpressing H1299 cells in a dose-dependent manner (e) and in co-overexpressing H1299 cells (f). Data in (a), (c), (d), (e) and (f) represent the mean of three independent experiments ± SEM. * P < 0.05,** P < 0.01.