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. 2017 Jul 28;7:6847. doi: 10.1038/s41598-017-07126-7

Figure 6.

Figure 6

c-Myb is associated with lung cancer progression because of regulation of DDR2 expression. (a) Heat map representation of a TCGA pan-cancer RNA-Seq dataset from the Cancer Browser (Illumina HiSeq) showing the MYB, DDR2, and EP300 expression levels in various cancer tissues. BL, bladder; BR, breast; CX, cervix; CL, colon; EM, endometrium; GL, glioma; HN, head and neck; KN, kidney; LV, liver; MN, melanoma; OV, ovary; PC, pancreas; AG, adrenal gland; PR, prostate; RT, rectum; SC, sarcoma; TT, testis; TY, thyroid. (b) An Oncomine boxed plot showing MYB expression levels in human breast, colon, ovary, prostate, and lung normal and cancer tissues. N, normal; C, cancer. (c) Western blot analysis of DDR2, c-Myb, phospho (p)-ERK, and ERK in H1299 cells expressing mock or anti–c-Myb shRNA. KD: c-Myb knockdown. (d) qRT-PCR analysis of DDR1. DDR2, TWIST1, ACTA2, CDH2, VIM, CDH1, and MYB under the conditions shown in panel c. Data represent the mean of three independent experiments ± SEM. * P < 0.05, ** P < 0.01. (e) Western blot analysis of DDR2, c-Myb, pERK, and ERK in H1299 cells expressing mock or anti–c-Myb shRNA, or analysis of ectopically expressed DDR2 in H1299 cells expressing anti–c-Myb shRNA. (f,g) Quantification of proliferation (f) and invasion (g) shown by H1299 cells under the conditions shown in panel e. Data represent the mean of three independent experiments ± SEM. * P < 0.05,** P < 0.01. (h) qRT-PCR analysis of DDR2, TWIST1, ACTA2, CDH2, VIM, CDH1, and MYB under the conditions shown in panel e. Data represent the mean of three independent of three experiments ± SEM.; * P < 0.05, ** P < 0.01, # P < 0.01 as compared to c-Myb knockdown groups. (i) Immunostaining of N-cadherin and nuclei in H1299 cells under the conditions shown in panel e. The GFP signal indicates the cells ectopically expressing DDR2.