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. 2017 Jul 28;7:6796. doi: 10.1038/s41598-017-07179-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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cdk9 is necessary for glial survival. (a–h) Representative images of 7d old adult brains immunostained with anti-Repo to label glia for Fig. 1i,j. The images are maximum projections of optical sections. The outlined area demarcates the region of the central brain where the number of glia were quantified. (i) Number of anti-Repo-expressing glia in the central brain of 7d old adult flies in mir-31a mutants (mir-31a KO/KO) and where UAS-GFP, UAS-CG16947, UAS-cdk9 RNAi or UAS-p53 RNAi lines were expressed in glia using Repo-Gal4. Glia were represented as a percentage of the control animals, Repo-Gal4 > UAS-GFP (left axis). Raw number of Repo-expressing glia in the central brain of the genotypes (right axis). One-way ANOVA was used for statistical analysis and error bars represent SEM. (j) Number of anti-Repo-expressing glia in the central brain of 7d old adult flies. UAS-GFP, UAS-CG16947 and UAS-cdk9 RNAi expression was driven by Repo-Gal4 under the control of tubulin Gal80^ts (tubGal80^ts). Adult-only expression was achieved by rearing the flies at 18 °C then moving the flies to 29 °C to allow Repo-Gal4 activity after eclosion. Data is represented as a percentage of the number of glia in the UAS-GFP control condition (left axis). Raw number of Repo-expressing glia in the central brain of the genotypes (right axis). One-way ANOVA was used for statistical analysis and error bars represent SEM.