Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 28;7:6838. doi: 10.1038/s41598-017-07144-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assessing enhanced invasion potential and lineage tracing. (a) CAFLC invasion ability was measured using Matrigel-coated transverse inserts. Invasive cells were stained with Giemsa and quantitatively analyzed in several fields of view. The experiments were conducted in triplicate. Scale bar represents 200 μm. The graph indicates quantitative results by counting invasive cells. CSCcmT47D-CAFLCs and CSCcmBT549-CAFLCs were more invasive than control miPS-fibroblasts. The data were analyzed using an unpaired two-tailed Student’s t-test and are presented as the mean ± standard deviation. **P < 0.01, *P < 0.05. (b) In situ hybridization analysis using a probe targeting GFP (red) and immunofluorescence analysis of FSP1 (green). The strong GFP signal in the nucleus indicated that CAFs retained plasticity. Scale bar represents 10 μm.