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. 2017 Jul 28;7:6786. doi: 10.1038/s41598-017-06988-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examination of the fluoride resistance of E. cloacae FRM-Δp1ΔIs123 (elimination of plasmid p1 and the three genomic islands) and E. cloacae FRM-Δp1 (elimination of plasmid p1). (a) Four strains were streaked onto Burk plates without fluoride (upper) and with 1,000 mg/L fluoride (lower). (b) The minimum inhibitory concentration (MIC) of fluoride for E. cloacae FRM, E. cloacae FRM-Δp1ΔIs123, E. cloacae FRM-Δp1, and E. cloacae FRM-ΔIs3G.