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. 2017 Jul 28;7:6794. doi: 10.1038/s41598-017-06354-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology and proliferation of osteoblasts on different substrates: (a) Live/dead staining for cell after seeding of 24 h, green fluorescence indicating cells alive while the red visualizing dead cells; (b) Morphology and of osteoblasts on glass, PDLLA, P-PUU-1, P-PUU-2, and P-PUU-3 by CLSM at 24 h; (c) cell spreading areas (n = 200) after seeding of 24 h; (d) Cell proliferation by MTT at 4, 7, 14 and 21 days (# means P < 0.05, compared with glass groups; * means P < 0.05, compared with PDLLA groups; Δ means P < 0.05, compared with P-PUUs groups).