. 2017 Jul 28;7:6794. doi: 10.1038/s41598-017-06354-1

Table 2.

Oligonucleotide primers for gene expression analysis in RT-PCR.

Gene	Primer sequence (F = forward, R = reverse)	Length of product/bp	Accession no.
COL I	F5′GTCTTCCTGGTGAATTCGGT3′	102 bp	NM_007743
COL I	R5′TTCCAATAGGACCAGAAGGG3′	102 bp	NM_007743
OPN	F5′CTTTCACTCCAATCGTCCCTAC3′	165 bp	NM_009263
OPN	R5′CCTTAGACTCACCGCTCTTCAT3′	165 bp	NM_009263
Cbfα-1	F5′AGTTCCCAAGCATTTCATCC3′	145 bp	AF_010284
Cbfα-1	R5′GGCAGGTACGTGTGGTAGTG3′	145 bp	AF_010284
β-actin	F5′GGTCATCACTATTGGCAACG3′	72 bp	NM_007393
β-actin	R5′GGCAGGTACGTGTGGTAGTG3′	72 bp	NM_007393

Collagen I (COL 1), osteopontin (OPN) and core-binding factor α-1 (Cbfα-1).