Table 1.

Oligonucleotides used for site-directed mutagenesis or final product sequencing.

Mutagenesis primers	Oligonucleotide sequence
E193D	5′-GGC TCA GAC ATG CAA GA C GTC ATT GGC TCA GCC-3′
E193A	5′-GGC TCA GAC ATG CAA G C A GTC ATT GGC TCA GCC-3′
M294C	5′-GTG GGA GCT GTC ATC TGC CCA CAC AAC ATG TAC-3′
M294Q	5′-GTG GGA GCT GTC ATC CA G CCA CAC AAC ATG TAC-3′
N472Q	5′-G ATG AAT GAC TTT CTG C A G GTT CTA CAG AGC TTA C-3′
N472A	5′-G ATG AAT GAC TTT CTG C A G GTT CTA CAG AGC TTA C-3′
Sequencing primers	Oligonucleotide sequence
IRES-for	5′-TAGGCGTGTACGGTGGG-3′
IRES-rev	3′-TATAGACAAACGCACACCG-5′
M13-for	5′-TGTAAAACGACGGCCAG-3′
M13-rev	3′-CAGGAAACAGCTATGAC-5′

Mutated triplet is shown in bold, while the substituted amino acids from the Wt hDMT1 cDNA sequence are underlined. IRES primers were used to sequence pIRES2-DsRed clones, while M13 primers were used to sequence Pol1 clones.