Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 28;7:6771. doi: 10.1038/s41598-017-06964-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

In ovo blocking of the Fgf8 signal causes abnormal Sema3c expression and migration defects in cardiac neural crest cells. (A) Injection of Fgf8 neutralizing antibody (Fgf8ab) led to abnormal ectopic enhancement of SEMA3C expression in the pharyngeal arch region (white circle) in HH22 chick embryos. (B) SEMA3C mRNA expression in the pharyngeal arch region of HH22 chick embryos (control) and those injected with Fgf8ab (n = 6 per group). (C) Pharyngeal arch regions of chick-quail chimeras with bilateral transplantation of cNCCs. Fgf8ab injection causes less migration of cNCCs from the 6th pharyngeal arch region (white arrowhead) to the outflow tract (black arrowhead) compared to control. Thirteen chimeras and thirteen control embryos were analyzed. (D) India ink injection demonstrated hypoplasia of the 6th pharyngeal arch artery in Fgf8ab injected embryos (arrow) compared to control. v, ventricle; oft, outflow tract; III, IV and VI, 3rd, 4th, and 6th pharyngeal arch arteries, respectively; Paa, pharyngeal arch arteries. Scale bars, 500 μm. **p < 0.01. Error bars represent s.e.m.