Table 1.
Potential subsite cooperativity analysis for KLK7.
| Fixed residue | Affected residue(s) | Change (percentage-points) | Vice-Versa change |
|---|---|---|---|
| P3_A | P1prime_A | 11.6 | 11.4 |
| P3_A | P2_V | 16.4 | 16 |
| P3_K | P2_K | 11.5 | 12.5 |
| P3_N | P1prime_A | 18.6 | 11.2 |
| P3_Q | P1prime_R | 13.4 | 12.4 |
| P3_Q | P2_K | 10.8 | 12.2 |
| P3_Q | P3prime_D | 17.1 | 14.6 |
| P3_Q | P3prime_V | 18.2 | 11.1 |
| P3_V | P2_P | 22 | 14.9 |
| P3_V | P3prime_N | 10.6 | 19.1 |
| P2_K | P1prime_S | 19.3 | 12.3 |
| P2_K | P2prime_N | 14.8 | 20.8 |
| P2_V | P1_F | −12.7 | −10.3 |
| P2_V | P1prime_A | 11.1 | 11.1 |
| P1_Q | P3prime_I | 15.6 | 11.3 |
| P1prime_H | P2prime_M | 21.3 | 19.7 |
| P1prime_R | P3prime_V | 15.9 | 10.5 |
| P2prime_N | P3prime_I | 25.1 | 13 |
Potential subsite cooperativity analysis for 353 KLK7 cleavage sites detected in the GluC library was calculated using the CLIP-PICS web server (http://clipserve.clip.ubc.ca/pics)18. Minimum difference for subsite dependency was set to ±10 percentage-points. Subsite dependency was checked for all positional occurrences >1 × natural abundance and restricted to P3-P3′. Subsites with a change ≥15 percentage-point are bold.