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. 2017 Jul 28;37(16):e00001-17. doi: 10.1128/MCB.00001-17

FIG 2.

FIG 2

Regulation of angiogenic activities by let-7. (A) Relative expression of let-7 family members let-7b, let-7c, and let-7d in HUVECs after let-7 anti-miR transfection as detected by qPCR. U6 served as a normalization control. An anti-let-7 sequence is shown. **, P < 0.01. The relative expression of let-7d after Ad-let-7a/d/f or Ad-LacZ infection in HUVECs was detected by qPCR. U6 served as a loading control. ***, P < 0.001. (B) Representative pictures of in vitro Matrigel assays after Ad-let-7 or Ad-LacZ infection or after anti-let-7 or control anti-miR transfection in HUVECs. (C) Quantification of branching point per field in panel B. (D) Quantification of VEGF-induced proliferation indicated by BrdU incorporation after Ad-let-7 and anti-let-7 transfection in HUVECs. ***, P < 0.001. (E) Representative pictures of migration assays in response to VEGF treatment after Ad-let-7 and anti-let-7 transfection in HUVECs. (F) Quantification of the migration distance (in micrometers) depicted in panel E.